Marko Bjelaković, Dušan Sokolović, Milica Bjelaković

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Polyamines, spermine, spermidine, and putrescine are ubiquitous in living cells. Polyamines play important roles in cell growth, proliferation, and survival. Our aim was to determine the concentration of the spermine, spermidine, and putrescine in the rat liver during the first 6 months of postnatal life. A total of 45 albino Wistar rats, maintained under controlled temperature (20±2°C) in the animal room facilities were included in this study. On the day 1, 3.5 months, and 6 months of postnatal life, rats were sacrificed by cervical dislocation. Thr liver was removed and washed in the 0.9% solution of sodium chloride. Concentrations of spermine, spermidine, and putrescine were determined. Concentration of polyamines in the liver tissue of the Wistar albino rats aged 1 day, 3.5 months, and 6 months was respectively: spermine (Sp) (33.81 ± 3.04; 128.15 ± 6.62; 74.34 ± 1.12 mg/g of wet weight); spermidine (Spd) (121.92 ± 6.23; 53.34 ± 3.31; 56.32± 1.41 mg/g wet weight); and putrescine (Put) (8.92 ± 0.98; 9.37 ± 0.98; 20.93 ± 1.15 mg/g wet weight). Polyamine concentration in the rat liver fluctuated during postnatal life. The concentration of the spermidine was highest in the rat liver on the first postnatal day, much higher than spermine, this ratio inverted in 3.5 months old rats. The concentrations of the spermidine and spermine were almost equal at the 6 months of the postnatal life. The concentration of putrescine steadily increases during postnatal life.


spermin, spermidin, putrescine, rat liver, postnatal life

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