PRODUCTION OF NIGERICIN AND NIPHIMYCIN BY SOIL ISOLATE STREPTOMYCES SP. MS1: ANTI-CANDIDA BIOASSAY GUIDED RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED CULTURE MEDIUМ

Marija Mojićević, Jovana Grahovac, Miloš Petković, Ivan Vučković, Jelena Dodić, Siniša Dodić, Sandra Vojnović

DOI Number
10.2298/FUPCT1701001M
First page
001
Last page
016

Abstract


Anti-Candida bioassay guided optimization of the culture medium was used in order to enhance the antifungal activity of the soil isolate MS1. Its morphological, physiological and biochemical characteristics, as well as 16S rDNA sequencing, assigned an MS1 isolate to genus Streptomyces. Optimization of the culture medium was achieved by experimental factorial design and response surface methodology. Maximal antifungal components production was obtained with starch, soybean meal and phosphates content of 40.52, 5.10 and 2.21 g/L, respectively. Chromatography and 1H and 13CNMR spectroscopy were employed for purification and structural characterization of antifungal antibiotics concurrently produced by this strain. These antibiotics were identified as polyether carboxylic antibiotic nigericin and guanidyl-polyol macrolide, niphimycin.

Keywords

antifungal, nigericin, niphimycin, Streptomyces sp., media optimization

Full Text:

PDF

References


Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., Nucleic Acids Res., 25, 3389-402.

Bogaert, C., Jouany, J.P., Jeminet, G., 1990. Animal Feed Sci Tech., 28, 183-97. doi: 10.1016/0377-8401(90)90152-X

Brady, D., Lowe, N., Gaines, S., Fenelon, L., McPartlin, J., O’Farrelly, C., 2003. J Food Sci., 68, 1433-7. doi: 10.1111/j.1365-2621.2003.tb09662.x

Cole, J.R., Wang, Q., Cardenas, E., Fish, J., Chai, B., Farris, R.J., Kulam-Syed-Mohideen, A.S., McGarrell, D.M., Marsh, T., Garrity, G.M., Tiedje, J.M., 2009. Nucleic Acids Res., 37, 141-5. doi: 10.1093/nar/gkn879

Cross, T., 1989. Growth and Examination of Actinomycetes-Some Guidelines. In: Williams ST, Sharpe ME, Holt JG, editors. Bergey' s Manual of Systematic Bacteriology Vol 4. Baltimore, MD: Williams & Wilkins Company. p. 2340-3.

David, L., Ayala, L.H., Tabet, J.C., 1985. J Antibiot., 38, 1655-63. doi: 10.7164/antibiotics.38.1655

Fang, A., Wong, G.K., Demain, A.L., 2000. J Antibiot., 53, 158-62. doi: 10.1038/ja.2010.71

Fruh, T., Chemla, P., Ehrler, J., Farooq, S., 1996. Pestic Sci., 46, 37-47. doi: 10.1002/(SICI)1096-9063(199601)46:1<37::AID-PS339>3.0.CO;2-K

Gesheva, V., Ivanova, V., Gesheva, R., 2005. Microbiol Res., 160, 243-8. doi: 10.1016/j.micres.2004.06.005

Grabley, S., Hammann, P., Raether, W., Wink, J., Zeeck, A., 1990. J Antibiot., 43, 639-47. doi: 10.7164/antibiotics.43.639

Grahovac, J., Grahovac, M., Dodic, J., Bajic, B., Balaz, J., 2014. Crop Prot., 65, 143-52. doi: 10.1016/j.cropro.2014.07.020

Herlich, K., 1990. Official Methods of Analysis of the Association of Official Analytical Chemists Vol 5. Association of Official Analytical Chemists. Arlington, MD.

Ivanova V., Schlegel, R., Dornberger, K., 1998. 57-13. J Basic Microbiol., 38, 415-9. doi: 10.1002/(SICI)1521-4028(199811)38:5/6<415::AID-JOBM415>3.0.CO;2-Z

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F., Hopwood, D.A., 2000. Practical Streptomyces Genetics. The John Innes Foundation. Norwich, England.

Kim, H.Y., Kim, J.D., Hong, J.S., Ham, J.H., Kim, B.S., 2013. J Basic Microbiol., 53, 581-9. doi: 10.1002/jobm.201200045

Lane, D., 1991. 16S/23S rRNA sequencing. In: Stackebrandt E and Goodfellow M, editors. Nucleic acid techniques in bacterial systematic. John Wiley and Sons, Inc. Chichester, UK, p. 115-75.

Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., Higgins, D.G., 2007. Bioinformatics., 23, 2947-8. doi: 10.1093/bioinformatics/btm404

Li, X.B., Zhao, G.R., Zheng, H., Yuan, Y.,J, 2007. Process Biochem., 42, 662-8. doi: 10.1016/j.procbio.2006.12.007

Mouslim, J., Cuer, A., David, L., Tabet, J.C., 1995. J Antibiot., 48, 1011-4. doi: 10.7164/antibiotics.48.1011

Nakayama, K., Yamaguchi, T., Doi, T., Usuki, Y., Taniguchi, M., Tanaka, T., 2002. J Biosci Bioeng., 94, 207-11. doi: 10.1016/S1389-1723(02)80151-7

Nikodinovic, J., Barrow, K.D., Chuck, J.A., 2003. Biotechniques, 35, 932-6

Ogita, A., Matsumoto, L., Fujita, K., Usuki, Y., Hatanaka, Y., Tanaka, T., 2007. J Antibiot., 60, 27-35. doi: 10.1038/ja.2007.65

Porter, N., 1985. Pestic Sci.,16, 422-7. doi: 10.1002/ps.2780160419

Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.

Sanchez, S., Demain, A.L., 2002. Enzyme Microb Tech., 31, 895-906. doi: 10.1016/S0141-0229(02)00172-2

Singh, N., Rai, V., 2012. Int J Pharm Pharm Sci., 4, 94-101.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., 2013. Mol Biol Evol., 30, 2725-9. doi: 10.1093/molbev/mst197

Usuki, Y., Matsumoto, K., Inoue, T., Yoshioka, K., Iio, H., Tanaka, T., 2006. Bioorg Med Chem Lett.,16, 1553-6. doi: 10.1016/j.bmcl.2005.12.024

Westley, J.W., 1981. Antibiotics IV Biosynthesis. Springer-Verlag, New York.

Williams, S.T., Goodfellow, M., Alderson, G., Wellington, E.M.H., Sneath, P.H.A., Sackin, M.J., 1983. J Gen Microbiol., 129, 1743-813. doi: 10.1099/00221287-129-6-1743

Wu, Z., Bai, L., Wang, M., Shen, Y., 2009. Chem Nat Compd., 45, 333-7. doi: 10.1007/s10600-009-9350-x

Yarbrough, G.G., Taylor, D.P., Rowlands, R.T., Crawford, M.S., 1993. J Antibiot., 46, 535-44. doi: 10.7164/antibiotics.46.535


Refbacks

  • There are currently no refbacks.


ISSN 0354-4656 (print)

ISSN 2406-0879 (online)