FERMENTATION ENHANCES THE ANTIPROLIFERATIVE ACTIVITIES OF ANNONA SQUAMOSA SEED POLYSACCHARIDES ON HUMAN MCF-7 AND RHABDOMYOSARCOMA

Christianah Adebimpe Dare, Bolaji Bosede Oluremi, Toluwanimi Emmanuel Akinleye, Johnson Adekunle Adeniji, Moses Olubusuyi Adewumi, Oluokun Oluboade Oyedapo

DOI Number
https://doi.org/10.22190/FUMB240126002D
First page
001
Last page
007

Abstract


Cancer is a primary cause of death globally and a significant public health concern. Polysaccharides are complex carbohydrates found abundantly in natural sources, including plants, and have attracted interest due to their possible health advantages, including anticancer activities. Annona squamosa, also known as the sugar apple, has long been prized for its healing properties, thus an intriguing candidate for investigating its potential effects on cancer cells. Through boosting bioavailability, producing active metabolites, enriching the nutrient profile, altering the composition of bioactive substances, and influencing the gut microbiota, fermentation plays a crucial role in promoting the antiproliferative effects of food. Polysaccharide was extracted from the fermented and unfermented cotyledon and coat of A. squamosa seed, characterised by HPLC, and antiproliferative activity was investigated using the MTT assay on human breast adenocarcinoma (MCF-7) and rhabdomyosarcoma (RD) cell lines. The Vero cell line obtained from the kidney of a green monkey of African descent was used for selectivity. The polysaccharides displayed antiproliferative activity against the cancerous cell lines MCF-7 (breast cancer) and RD (rhabdomyosarcoma-soft tissue sarcoma), with IC50 values ranging from 27.10 ± 0.61 to 57.01 ± 0.06 µg/ml and a good selectivity for the cancer cells over normal body cells (Vero), with fermented better than unfermented. In conclusion, A. squamosa seed polysaccharides exhibited antiproliferative properties on MCF-7 and RD, which could be explored in  developing  a novel drug in cancer prevention and treatment.


Keywords

Annona squamosa, cancer cell lines, fermentation, polysaccharides, antiproliferative activity

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References


World Health Organization. Cancer, https://www.who.int/news-room/fact-sheets/detail/cancer/; 2022 [accessed 29 September 2023].

Guo R, Chen M, Ding Y, et al. Polysaccharides as potential anti-tumor biomacromolecules - A review. Front Nutr 2022; 9:838179.

Corso CR, Mulinari Turin de Oliveira N, Moura Cordeiro L, et al. Polysaccharides with antitumor effect in breast cancer: A systematic review of non-clinical studies. Nutr 2021; 13(6):2008.

Khan T, Date A, Chawda H, Patel K. Polysaccharides as potential anticancer agents—A review of their progress. Carbohydr Polym 2019; 210:412–428.

Al Kazman BSM, Harnett JE, Hanrahan JR. Traditional uses, phytochemistry and pharmacological activities of Annonacae. Mol (Basel, Switzerland) 2022; 27(11):3462.

Miao Y, Xu X, Yuan F, et al. Four cytotoxic annonaceous acetogenins from the seeds of Annona squamosa. Nat Prod Res 2016; 30(11):1273–1279.

Cheng-Yao MA, Jia-Hui LU, Xiang LI, Xiao LIU, Jian-Wei Chen. Eight new cytotoxic annonaceous acetogenins from the seeds of Annona squamosa. Chin J Nat Med 2019; 17(4):291-297.

Shehata MG, Abu-Serie MM, Abd El-Aziz NM, El-Sohaimy SA. Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits. Sci Rep 2021; 11(1):6224.

Zhao YS, Eweys AS, Zhang JY, et al. Fermentation affects the antioxidant activity of plant-based food material through the release and production of bioactive components. Antioxid 2021; 10(12):2004.

Sinaga DP, Tampubolon DKA, Kembaren RF, Martgrita MM. Fermentation process effect to enhance antioxidant and antibacterial activity of phenolic compounds and its possible application to galactomannan polysaccharides: A review. In IOP Conference Series: Earth Environ Sci 2022; 1097(1):012027.

Shabbir U, Tyagi A, Ham HJ, Elahi F, Oh DH. Effect of fermentation on the bioactive compounds of the black soybean and their anti-Alzheimer’s activity. Front Nutr 2022; 9:880361.

Dare CA, Oyedapo OO. Antioxidant and anti-inflammatory activities of polysaccharides from the coat of fermented and unfermented sugar apple (Annona squamosa L.) seed. Adv Mater Lett 2023; 14(3):23031729.

Liu H, Fan Y, Wang W, et al. Polysaccharides from Lycium barbarum leaves: Isolation characterization and splenocyte proliferation activity. Int J Biol Macromol 2012; 51:417–422.

Dare CA, Oyedapo OO, Akinlalu AO, Komolafe IJ, Fajobi AO, Ogunsusi M. Genotoxic activities of polysaccharides from cotyledon and coat of fermented and unfermented Annona squamosa L. seed. Egypt Acad J Biol Sci H Bot 2021; 12(1):189-207.

Yan J, Shi SS, Wang HW, Liu RM, Li N, Chen YL. Neutral monosaccharides composition analysis of plant-derived oligo- and polysaccharides by high performance liquid chromatography. Carbohydr Polym 2016; 136:1273–1280.

Rahman KW, Sarkar FH. Inhibition of nuclear translocation of nuclear factor-{kappa}B contributes to 3,3’-diindolylmethane-induced apoptosis in breast cancer cells. Cancer Res 2005; 65(1):364–371.

Rashidi M, Seghatoleslam A, Namavari M, et al. Selective cytotoxicity and apoptosis-induction of Cyrtopodion scabrum extract against digestive cancer cell lines. Int J Cancer Manag 2017; 10(5):e8633.

Kaczmarska KT, Chandra-Hioe MV, Zabaras D, Frank D, Arcot J. Effect of germination and fermentation on carbohydrate composition of Australian sweet lupin and soybean seeds and flours. J Agric Food Chem, 2017; 65(46):10064–10073.

Wang J, Hu Y, Wang D, Liu J, Zhang J, Abula S. Sulfated modification can enhance the immune-enhancing activity of Lycium barbarum polysaccharides. Cell Immunol 2010b; 263(2):219223.

Meng LZ, Lv GP, Hu DJ, et al. Effects of polysaccharides from different species of Dendrobium (Shihu) on macrophage function. Mol 2013; 18(5):5779–5791.

Gregoriou G, Neophytou CM, Vasincu A, et al. Anti-cancer activity and phenolic content of extracts derived from Cypriot Carob (Ceratonia siliqua L.) pods using different solvents. Mol 2021; 26(16):5017.

Agena R, de Jesús Cortés-Sánchez A, Hernández-Sánchez H, Jaramillo-Flores ME. Pro-apoptotic activity of bioactive compounds from seaweeds: promising sources for developing novel anticancer drugs. Mar Drugs 2023; 21(3):182.

Calderón-Montaño JM, Martínez-Sánchez SM, Burgos-Morón E, et al. Screening for selective anticancer activity of plants from Grazalema Natural Park, Spain. Nat Prod Res 2019; 33(23):3454–3458.

Siew Y, Yew H, Neo S, Seow S, et al. Evaluation of antiproliferative activity of medicinal plants used in Asian traditional medicine to treat cancer. J Ethnopharmacol 2020; 235:7587.

Shahbazi B, Najafabadi ZS, Goudarzi H, Sajadi M, Tahoori F, Bagheri M. Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells. J Venom Anim Toxins incl Trop Dis 2019; 25:e20190009.

Ogbole OO, Segun PA, Adeniji AJ. In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts. BMC Complement Altern Med 2017; 17:494.

Bezvin C, Tomasi F, Lohezie-Le D, Boustie J. Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. Phytomed 2003; 10(6–7),499–503.

Wang J, Luo J, Zha X, Feng B. Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl. Carbohydr Polym 2010a; 79(1):114–118.

Xu F, Liao K, Wu Y, Pan Q, Wu L, Jiao H. Optimization, characterization, sulfation, and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter. Carbohydr Polym 2016; 151:364–372.




DOI: https://doi.org/10.22190/FUMB240126002D

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